An alternative method for inactivating heteroagglutinins in human sera applicable to rubella haemagglutination inhibition testing at low dilutions.
نویسنده
چکیده
Serum agglutinins of chick and pigeon cells are predominantly immunoglobulin M and can be inactivated by 2 mercaptoethanol. 2 Mercaptoethanol is more effective than strong suspensions of red cells in removing the agglutinins of indicator cells from sera being prepared for HAI tests. In rubella HAI tests from a dilution of 1 to 10 dilute 2 mercaptoethanol offer a convenient method of removing agglutinins, and, at higher concentration, allow dilutions of sera from 1 in 2-5 to be tested without significant interference by heteroagglutinins. HAI titres are comparable when red cell absorbed and 2 mercaptoethanol treated sera are tested in parallel.
منابع مشابه
Comparison of radial haemolysis with haemagglutination inhibition in estimating rubella antibody.
Radial haemolysis (RH) for assaying rubella antibody was studied in parallel tests with haemagglutination-inhibition (HAI) on 1102 sera from adult women. Simple to perform and economical in reagents, the RH test is as sensitive as the HAI test and is a useful method of screening antenatal and other sera for rubella immunity. It detects IgG but not IgM antibody.
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متن کاملAn assessment of radial haemolysis in the detection of rubella antibody.
The results obtained by radial haemolysis in the detection of antibodies to rubella virus compared well with those obtained by haemagglutination inhibition. Radial haemolysis is unaffected by non-specific inhibitors. The sera do not therefore require pretreatment and the results are less equivocal. Radial haemolysis appears to be as sensitive as immunofluorescence and floatation centrifugation....
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عنوان ژورنال:
- Journal of clinical pathology
دوره 29 5 شماره
صفحات -
تاریخ انتشار 1976